• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Methods of producing glucosone which comprises enzymatically oxidizing glucose with glucose-2-oxidase in a first zone and separating the concomitantly produced hydrogen peroxide from said first zone through a semi-permeable membrane into a second zone, said membrane being permeable only to compounds of a molecular weight of less than about 100.
    公开号:SU1291028A3
    申请号:SU823399998
    申请日:1982-02-17
    公开日:1987-02-15
    发明作者:А.Марселли Джон；О.Хорват Роберт
    申请人:Стандарт Брэндз Инкорпорейтед (Фирма)；
    IPC主号:
    专利说明:

    food ways
    ten
    15
    The invention relates to the industry, namely the production of fructose from glucose.
    The purpose of the invention is to increase the purity of the fructose obtained from glucose.
    The method of producing fructose is that when it is obtained from glucose by oxidation of the latter to glucosone with enzyme preparations with glucose-2-oxidase activity, hydrogen peroxide is formed in the oxidation reaction, and catalase is immobilized on diethylaminoethylcellulose. The zones of the reactor that oxidize glucose are reduced. reduction of hydrogen peroxide, membrane separated. permeable to hydrogen peroxide and impermeable to glucosone. As a result of using such a 20 reception, hydrogen peroxide, which is formed during the oxidation of glucose, diffuses through the membrane into the neighboring zone and is reduced there by immobilized catalase. The glucose produced during the reaction does not contain any impurities and can be reduced to fructose in the usual way using Rene nickel. The resulting preparations of fructose do not require additional purification, which is usually necessary for the production of fructose from glucose by oxidizing it to glucosone using an enzymatic system to remove hydrogen peroxide 35, but the non-separated semipermeable membrane from the oxidation zone. The fructose obtained in this way contains halogen hydrides and alcoholic fluorides, which load the product.
    Example: Into a 10 L reactor equipped with a glucosone impermeable membrane and permeable to hydrogen peroxide, add 3 liters
    . 25
    thirty
    40
    2.5% glucose solution, 05M45 by René nickel,
    The LIW is separated from the solution in the first zone, with more than 99% of the glucose converted into glucosone. The resulting bright glucosone solution was hydrogenated with the help of the Rene skeletal nickel analyzer under the pressure of gaseous hydrogen of 500 atm and temperature. The aqueous mixture is filtered by filtration from a catalyst, decolorized with an activated carbon, deionized on a cation and anion ion exchanger and concentrated, resulting in the crystallization of fructose. Fructose is obtained in the form of a food grade food crystal with a long stroke. The membrane used in the reactor ensures the passage of molecules with a molecular mass of less than 50 daltons. Similar results are obtained when using the following microorganisms of Polyporus obtusus, Radulura cascaru Zenzites trabea, Irpex glanus, Polus versicolar, Pellicularia gila mentosa, afmillaria mellea, Schizophuleum conmune, Corticumus causa, as the source of the activity of glucose oxidase.
    Thus, the use of the proposed method allows the production of fructose, which is not contaminated by mixtures of highly toxic substances, without the use of special stages of purification.
    权利要求:
    Claims (1)
    [1]
    Invention Formula
    A method for producing fructose from glose, which involves the oxidation of glucose to glucose by enzyme preparations containing glucose-2-oxase, the enzymatic reduction of hydrogen peroxide resulting from the oxidation of glucose, and the reduction of glucosone to fructose using
    NaF, in which 15 g of mycelium (dry weight) of Ondemanscilla mucipol is suspended. This suspension is in the same zone of the reactor, and the catalase suspended in 3 liters of water immobilized on diethyl aminoethylcellulose is placed in the next zone. The suspension in the first zone (oxidation) is stirred at
     about
    50
    so that, in order to increase the purity of the target product, glucose oxidation and enzymatic reduction of hydrogen peroxide were carried out in two zones separated by a membrane permeable to hydrogen peroxide and not permeable to the glitch zone, and to the zone for the enzymatic
    25 C and blown with oxygen. Simultaneously with the reduction of hydrogen peroxide, the suspension is mixed in a second -55 catalase, immobilized in a swarm zone. After 24 h of incubation of mycena with diethylaminoethyl cellulose.
    0
    five
    0 5.
    five
    0
    0
    5 by the use of Rene nickel,
    The LIW is separated from the solution in the first OH zone, with more than 99% of the glucose converted into glucosone. The resulting clear glucose solution is hydrogenated with a rene skeletal nickel catalyst under a hydrogen gas pressure of 500 atm and temperature. The aqueous mixture is filtered off from the catalyst, decolorized with activated carbon, deionized on a cationic and anionic ion exchanger and concentrated, resulting in the crystallization of fructose. Fructose is obtained as a food grade crystalline product in a high yield. The membrane used in the reactor allows the passage of molecules with a molecular weight of less than 50 daltons. Similar results are obtained when the following microorganisms are used as a source of glucose-2 oxidase activity: Polyporus obtusus, Radulura cascarum, Zenzites trabea, Irpex glanus, Polyperus versicolar, Pellicularia gilamentosa, afmillaria mellea, Schymulum conmuneeseezeeseezeezeeezeezeeezeezeeezeeezeeezeeezeezeeezeezeeezeeezeeezeeezeeezeeezeeezeeezeeezeeteeleezeemeseeleezee, Symporus versicolar, Zenzites trabea, Radiura cascarum, Afmillaria mellea, Schizophulum conmuneseezeeseeseeseezeemeezeeleezeemeselee, Polyperaus versicolar, Pellicularia gilamentosa, afmillaria mellea, Schizophulum conmureeseezeeseeseeseeseelee glenmentosa;
    Thus, the use of the proposed method makes it possible to obtain fructose, not contaminated with impurities of highly toxic substances, without the use of special purification steps.
    Invention Formula
    A method for producing fructose from glucose, which involves the oxidation of glucose to glucose by enzyme preparations containing glucose-2-oxidase, the enzymatic reduction of hydrogen peroxide formed during the oxidation of glucose, and the reduction of glucosone to fructose using René nickel,
    in order to increase the purity of the target product, the oxidation of glucose and the enzymatic reduction of hydrogen peroxide are carried out in two zones separated by a membrane permeable to hydrogen peroxide and not permeable to glucosone, and to the enzymatic zone
    the reduction of hydrogen peroxide is injected catalase immobilized on diethylaminoethyl cellulose.
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    同族专利:
    公开号 | 公开日
    FI69097B|1985-08-30|
    EP0056038A1|1982-07-21|
    JPS57500964A|1982-06-03|
    WO1981003664A1|1981-12-24|
    FI69097C|1985-12-10|
    AU543974B2|1985-05-09|
    FI820053L|1982-01-08|
    EP0056038B1|1984-09-26|
    US4321323A|1982-03-23|
    HU182404B|1984-01-30|
    AU7809081A|1982-01-07|
    EP0056038A4|1982-12-09|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    DE2008842A1|1969-02-27|1970-11-05|Kyowa Hakko Kogyo Co., Ltd., Tokio|Method and device for the continuous implementation of enzyme reactions|
    US3911140A|1973-07-02|1975-10-07|Milk Marketing Board|Preparation of concentrated starter cultures|
    DE2526558C2|1975-06-13|1984-11-29|Boehringer Mannheim Gmbh, 6800 Mannheim|Enzymatic analysis method|
    US4247641A|1979-05-29|1981-01-27|Cetus Corporation|Method for producing epoxides and glycols from alkenes|
    US4246347A|1979-05-29|1981-01-20|Cetus Corporation|Process for the production of fructose|US4423149A|1981-10-15|1983-12-27|Cetus Corporation|Process for the production of D-glucosone|
    US4442207A|1982-06-30|1984-04-10|Nabisco Brands, Inc.|Process for production of glucosone|
    US4440855A|1982-06-30|1984-04-03|Nabisco Brands, Inc.|Process for preparing L-glucosone|
    US4636464A|1983-03-11|1987-01-13|Kyowa Hakko Kogyo Co., Ltd.|Pyranose oxidase, method of making and method of use|
    US4568638A|1983-05-16|1986-02-04|Nabisco Brands, Inc.|Method for screening microorganisms for the production of glucose-2-oxidase|
    US4569910A|1984-02-24|1986-02-11|Cetus Corporation|Methods and reagents for pyranosone production|
    US4569913A|1984-02-24|1986-02-11|Cetus Corporation|P. obtusus pyranose-2-oxidase preparation|
    US4569915A|1984-02-24|1986-02-11|Cetus Corporation|P. obtusus strain|
    US4568645A|1984-02-24|1986-02-04|Cetus Corporation|Fungal pyranose-2-oxidase preparations|
    US5385827A|1989-09-20|1995-01-31|Clark; John R.|Method of geochemical prospecting|
    FR2801901B1|1999-12-07|2003-11-14|Roquette Freres|PROCESS FOR THE TRANSFORMATION OF ORGANIC MATERIALS, PARTICULARLY SACCHARIDES, COMPRISING AN ENZYMATIC OXIDATION STEP IN THE PRESENCE OF RUTHENIUM OR PALLADIUM|
    GB0306394D0|2003-03-20|2003-04-23|Univ Nottingham|Carnitine retention|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    US06/160,758|US4321323A|1980-06-18|1980-06-18|Carbohydrate process|
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